Uncover the dark secret behind the 400-year-old massacre of a Spanish expedition as Nathan Drake tracks the murderous trail of an ancient and secret Spanish sect. Caught in the middle of a rivalry between Jason Dante - his old friend - and Marisa Chase - the granddaughter of an archeologist who has mysteriously vanished without a trace - Nathan Drake delves into the treacherous river basins, undiscovered temple ruins and karst caverns of Central America in search of a legendary lost city, only to find himself pushed to the edge of the Golden Abyss

The events of Golden Abyss take place place prior to Uncharted: Drake's Fortune. Adventurer Nathan Drake accompanies old friend Jason Dante to a Panama dig site headed by the mistrustful Marisa Chase. At the site, they find the bodies of poisoned Spanish conquistadors, and a grave marker with a Visigoth symbol. After Dante has to leave due to a phone call, Marisa shows Drake an amulet she hid from Dante, inherited from her grandfather Vincent Perez and they agree to team up. Nathan and Marisa are then attacked and captured by Dante's real partner, warlord Roberto Guerro, but the pair escape. They go to Perez's house, who hired Dante to continue his research after a terminal cancer diagnosis; Dante paid off Guerro for access to the site in return for a share of the treasure that Guerro will use to fund his conflict, while Marisa went there to finish her family's work. The pair learn that the marker referred to the Sete Cidades, an ancient Christian sect dedicated to finding the Seven Cities of Gold. Friar Marcos de Niza, a member of the sect, had been part of a failed expedition led by Francisco Vásquez de Coronado to find the city of Quivira.